Sentence examples for single guide from inspiring English sources

And they altered the nucleotide code of this single guide RNA, redirecting Cas9 to cut elsewhere.

These applications require the design of single guide RNAs (sgRNAs) that are efficient and specific.

However, for multiple targets, independent expression of multiple single guide RNAs (sgRNAs) is needed, which is less convenient.

The single guide RNA (sgRNA) targets were obtained via the online program generated by Feng Zhang's laboratory (http://crispr.mit.edu/).edu/

Single guide RNAs (sgRNAs) targeting the Col9-a1 locus (KH.C8.248) were designed and tested essentially as described36,44.

To utilize Cas9, so-called single guide RNAs (sgRNAs) need to be designed for each target gene.

It requires a custom designed single guide-RNA (sgRNA), a Cas9 endonuclease, and PAM sequences in the target region.

Ryan, D. E. et al. Improving CRISPR Cas specificity with chemical modifications in single-guide RNAs.

We synthesized four single-guide RNAs (sgRNAs) targeting the conserved regions of HBV.

A chimeric single-guide RNA (sgRNA), the target sequence, directs the Cas9 nuclease to a specific cleavage site.

sgRNA, single-guide RNA; UTR, untranslated region; CAM, calmodulin promoter; PfU6, P. falciparum U6 promoter; hDHFR, human dihydrofolate reductase.