Although many treatment efficacy questions can best be answered by single focus studies, we recommend that such designs be adopted only after less restrictive designs are first considered.
Although genome-editing and shRNA technologies have not yet been tested in mosaic zebrafish cancer models, these techniques are amenable to both focused studies of single genes and large-scale genome-wide screens.
However, the use of these agents is still based on limited evidence, generally from small, single center studies just focused on their acute hemodynamic effects.
As the baseline assessment, this single cohort-focus study has the unique merit of gathering the initial fast-track batch, and the last group of post-registered attending the 3-year traditional generic program.
The majority of study samples included both male and female participants, and a single study focused exclusively on young women.
Moreover, only a single study focused on estimating dates of Tapirus divergence using sequence variation at the mitochondrial cytochrome oxidase II gene [ 18].
A highly focused, single objective study may require relatively few instruments while a broad-based, multi-faceted study will require numerous measures.
By comparison, a Tier C program is built around a single small study focused on MDR pathogens that are susceptible to the Tier C agent.
In Spain, the single large study focused on prenatal exposure to drugs of abuse was conducted in Barcelona (alcohol, tobacco and prescription drugs were not included); using meconium testing an overall positivity of 10.9% for any drug of abuse was shown [ 12].
Most importantly, risk factors described in this study have been previously mentioned in studies restricted to single focus of infection or pathogen, by other authors from different parts of the world, suggesting important external applicability of these conclusions.
