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Remarkably, among the elongation and desaturation enzymes associated with LCPUFA synthesis, only a single elongation enzyme was differentially expressed.
For example, 60 probesets were characterized with single elongation events in both the GV-MII and GV-MIIpa analysis, including the probeset for transcripts of Arf6 (MGI:99435, Figure S6).
Consistent with this mechanism, experimental studies in baboon neonates have revealed that exposure to different levels of DHA in formulas induced global changes in gene expression in cerebral cortex [46], and only a single elongation enzyme was differentially expressed: the analogue to human ELOVL5.
On its own a short exposure to Chi consistently elicits a single elongation without clear protrusions or invaginations.
The SwfA proteins are each composed of only one complete PKS module, indicative of either an iterative mode of action or a single elongation.
18b This may be a difference between multisubunit RNAPs and DNAPs, because, on the basis of single elongation complex studies, DNAPs appear to oscillate freely and rapidly pre ↔ post.
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In doing so, the nanomechanical fingerprint for the conformational transition of carboxymethyl amylose at about 270 pN is used as an indicator for identifying the single chain elongation.
We hope that this line of research provides an example of using characteristic shoulder plateau of α-linked glycan to identify single chain elongation, allowing for direct measurement of the unwinding force of supramolecular structure using SMFS.
Interpreting these results remains challenging because multiple competing activities contribute to single filament elongation rates.
We also obtained qualitatively similar results by measuring telomere length after HOT1 and TCAB1 knockdowns 3 days post transfection by a universal, single telomere-elongation length analysis (STELA; Figure 6D F).
Nakamura et al. [ 21] suggested that sequence-specific interference favoring dephasing by inhibiting single-base elongation on the Illumina Genome Analyzer II is a factor of sequence coverage variability.
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