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Design: Prospective, single crossover design.
The designed algorithm can pick a single crossover site randomly.
A single crossover point on both parents' chromosome is selected.
T-S, single crossover mutant; T-D, double crossover mutant; T-N, wild-type pattern.
However, it is possible that a single crossover could have occurred at cbh1 gene.
We further found that the plasmid was integrated into the chromosome by single crossover.
Similar(18)
The mutants with kanamycin resistance were selected, and further verified by PCR with primers of Δ budC A single crossover-F and Δ budC A single crossover-R for crossover upstream, or Δ budC B single crossover-F and Δ budC B single crossover-R for crossover downstream.
Bands generated by single-crossover at the upstream homologous arm are indicated by asterisks.
Asterisks indicate bands generated by single-crossover at the downstream homologous arm.
We found three single-crossover clones with homologous recombination occurred only at the 5′ homologous arm.
One of the kanamycin-resistant transconjugants (single-crossover) was picked on LB plate containing 50 μg/ml kanamycin.
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