Sentence examples for single copy insertion from inspiring English sources

Using the full build service platform at Knudra Transgenics, the plasmids pNU164 and pNU191 were constructed by inserting daf-16 cargo into MosI-mediated single copy insertion (MosSCI) vector backbones.

Our results showed that No. 4 and No. 13 Rs-cps transgenic N. benthamiana plants had a single copy insertion.

Picture in (a-b) show representative pattern observed in four single copy insertion lines transformed in the Col-0 background per construct and (c) an untransformed Col-0 control.

Southern blot hybridization and inverse PCR analyses of genomic DNA demonstrated a precise piggyBac-mediated, single copy insertion of the pBac[3xP3-EGFP afm,Vg-DefA] transposon in each transgenic line.

DNA blot analysis showed single copy insertion of transgene in SH41.

The mobile genetic element ISEcp1, a single copy insertion sequence responsible for mobilization of bla genes and identified upstream of several blaCTX-M genes carried by E. coli and Klebsiella spp isolates, was found in association with all of our isolates.

The chance of acquiring multiple T-DNA insertion lines (51%) was similar to getting single copy-insertion lines (49%).

An improved Tn7-based system for the single-copy insertion of cloned genes into chromosomes of Gram-negative bacteria.

The respective selection marker cassettes were used to derive mini-Tn7 elements that can be used for single-copy insertion of genes into bacterial chromosomes.

GSC, germline stem cells; IGF-1, insulin-like growth factor; IP, immunoprecipitation; mosSCI, Mos1-mediated single-copy insertion; MS, mass spectrometry.

To detect the endogenously produced protein encoded by c30f12.4 and the location of C30F12.4 in worms, we constructed a 3×FLAG-GFP C30F12.4 transgene worm using the Mos1-mediated single-copy insertion (mosSCI) system.