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However, the proportion of sperm used for a single copulation differed between the two species (i.e., 8.4% in M. pruinosa and 32.5% in C. cornelia).
Males transfer few sperm during a single copulation (∼ 65 [35]; ∼142 [36]), and spermatophores are small [37] and unlikely to provide females with non-sperm resource benefits.
Given the low levels of female fertility and severe sperm limitation observed previously in this wild population [24], we asked whether a single copulation confers a significant reproductive advantage to a female by a) increasing fertility, and b) slowing the rate at which fertility declines when a female is housed in isolation.
Courtship and copulation were closely observed and a single copulation was permitted per female.
Thus, a considerable amount of stored accessory gland secrete was transferred during a single copulation.
Following a single copulation with a male, females produced on average 1.76 (SE = 0.16) fertilised egg-sacs.
Similar(39)
For long-tailed macaques, which have only single-mount copulations, copulations were scored if they involved three or more thrusts.
The following morning, within 2 hr of incubator "dawn," a single virgin male was aspirated into each vial containing a female assigned to the "mated" treatment and individual copulations were observed.
Since single-mount copulations are shorter than multiple-mount copulations, this may predict that long-tailed macaques will more readily use opaque objects to hide copulations than rhesus macaques.
Yet, rhesus macaques are multiple mounters, whereas long-tailed macaques often display single-mount copulations (Shively et al. 1982).
Males were fertile but, in courtship assays, they displayed a defect similar to that observed previously for tko 25t (Toivonen et al., 2001): in two separate experiments, only one of 11 and one of 13 individual sesB males reached copulation with single wild-type females, whereas ten out of ten wild-type males successfully copulated.
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