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All data were presented as means±SEM Student's t-test was used for single comparisons.
For single comparisons of two means (for example, energy intake at lunch), data were verified as normally distributed using a Shapiro Wilk test and paired t-tests were used for parametric data.
Significant differences between individual biological replicates in western blots were determined using the unpaired two-tailed Student's t-test for single comparisons or one-way ANOVA with Dunnett's multiple comparison test using Graph Pad Prism software.
Considering eight comparisons, a type I error probability = 0.00639 was used for the single comparisons.
Single comparisons were performed by the Student's paired t-test.
Paired t tests were used for single comparisons.
Single comparisons were performed by unpaired Student's t-test.
P-values of post-hoc single comparisons were Bonferroni corrected.
We also use either one-way ANOVA, or Wilcoxon-Mann-Whitney U tests for wild-type vs. mutant single comparisons.
Two-tailed paired t-tests were used whenever appropriate; otherwise unpaired t-tests were used for single comparisons.
The behavioral data were analyzed by two-way ANOVA, followed by Student t-test for single comparisons.
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