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Distances between cuts by the two degradations were found to be shorter than those within each set of sites, indicating a trend for a very broad "clustering", without however, a single coincidence of the two kinds of cuts.
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In addition to commonly analyzed single spectra, coincidence spectra or even two-dimensional (2-D) coincidence spectra can also be used in the MCLLS approach and may provide more accurate results.
A blood sampling flow-through detector can rely on detection of emitted positrons or single and/or coincidence detection of annihilation photons from blood in the catheter lumen.
10.7554/eLife.01434.004 Figure 2. Single-molecule coincidence and ALPHAScreen mapping of interactions among cavin1, 2 and 3. (A ) Histogram of single-molecule coincidence between cavin1-GFP and cavin2-Cherry co-transfected in MCF-7 cells.
An average of 1000 events was accumulated for each single-molecule coincidence histogram.
(B ) Histogram of single-molecule coincidence for cavin1-GFP and cavin1-Cherry after hypo-osmotic treatment.
(D ) Histogram of single-molecule coincidence between cavin2 and cavin1 after hypotonic treatment.
(B ) Histogram of single-molecule coincidence between CAV1-GFP and Cavin1-Cherry.
(F ) Histogram of single-molecule coincidence between cavin2 and cavin3 after membrane stretching.
(E ) Histogram of single-molecule coincidence between cavin3 and cavin1 after membrane stretching.
We performed dual colour co-transfection of MCF-7 cells and single-molecule coincidence analysis of all three cavins.
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