Sentence examples for single cells suspension from inspiring English sources

In order to rapidly lyse the moving cells and completely transport cellular contents into the detection channel, the angle of the side-flow channels, the asymmetric design of the channels, and the number, shape and layout of micro-obstacles were optimized for effectively redistributing and mixing the laminar flows of single cells suspension, cell lysing reagent and detection buffer.

Single cells suspension was washed and filtered through a 40 μm cell strainer (BD Biosciences, Milan, Italy).

In brief, the bone marrow cells were flushed from the long bone collected and prepared for single cells suspension.

Single cells suspension and monolayer cells were given sterile 1 to 20 μM final concentration of verapamil incubated for 30 minutes in 37°C.

The supernatant containing single cells suspension was collected into a fresh 15 mL falcon tube kept on ice, to which 2 mL Dulbecco's modified Eagle's medium (DMEM /F12 (1 : 1) medium (Gibco, Carlsbad, CA) supplemented with 20% fetal calf serum (Gibco) was added, and the digestion step was repeated three times.

MEFs were manually removed and iPSCs were brought to single cells suspension using Accutase Life Technologiess) and plated into a 48-well plate coated with polyornithine (100 μg/mL, Sigma Aldrich)/laminin (3 μg/mL, Invitrogen) at 25,000 cells per well, in mTeSR1 (Stem Cell Technologies) and 10 μM ROCKi.

Single cells suspensions were obtained by mechanical dissociation.

Single cell suspension was subjected to flow cytometry analysis.

And single cell suspension was prepared as described previously with small modifications (Ponti et al., 2005).

Single cell suspension of iPS cells was prepared by trypsinization.

Single cell suspension was prepared as previously described [18].