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Particularly, advances in single cell manipulation and amplification techniques bring sequencing technology to the single-cell level.
For the purpose of single cell analysis, single cell manipulation capabilities, such as positioning, immobilization, and sorting of target cells, are needed.
Wang et al. [24] reported development of a single cell manipulation tool integrating microfluidic technology with optical tweezers for high accuracy sorting of small cell populations (see Fig. 8).
Recent breakthroughs in single cell manipulation methods for the reconstitution of bioengineered tooth germ and the investigation of in vivo development of artificial tooth germ in the adult oral environment have been reported.
First, the ability to create patterns of co-cultured single cells is not possible with other higher-throughput techniques for single cell manipulation.
Challenges remain both to enable efficient extraction, isolation and patterning of single cells from heterogeneous solutions as well as to keep them alive during the process due to a limited degree of control over single cell manipulation.
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We believe that for single cell manipulations and measurements to be used effectively, tools must be developed to allow precise, individual control of multiple traps.
Along with microfluidics, these microdevices make single-cell manipulation possible with high spatial and temporal resolution.
Integrating single-cell manipulation techniques in traditional and emerging biological culture systems is challenging.
The serial micromanipulation technique was also used in combination with other parallel but less precise and specific approaches, to improve the overall efficiency and success rates for large-scale, single-cell manipulation operations.
Summary: Recent advances in single-cell manipulation technology, whole genome amplification and high-throughput sequencing have now made it possible to sequence the genome of an individual cell.
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