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Single cell imaging.
The technique offers perspectives for single cell imaging.
Third, we applied the new ruler for prognosis purposes to analyze CML lysates with high sensitivity instead of single cell imaging.
Thus, we examined the effects of CXCL12 pretreatment on NMDA-induced [Ca+2]i changes in cortical neurons by using fura-2-based single cell imaging.
DOI: http://dx.doi.org/10.7554/eLife.05808.009 Having verified the suitability of CaRuby-Nano for single cell imaging experiments in vitro and in vivo, we now set out to test CaRuby-Nano for imaging neuronal network activity when applying sensory stimulation.
Further improvement can be gained using additional hardware, e.g., dynamic retinal tracking, and software, e.g., registration/dewarping algorithms methodologies that have been successfully applied to other retinal imaging modalities for single cell imaging.
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Here, we combine flow cytometry and a quantitative live single-cell imaging approach to demonstrate how the controlled differentiation of mouse ESCs towards a primitive streak fate in culture results in cells displaying many of the characteristics observed during early mouse development including transient brachyury expression, EMT and increased motility.
Using single cell-imaging methods we have found that the volume of adherent cells grown in culture decreases as the cells rounds when it enters mitosis.
Single-cell imaging was repeated for six cells, and each cell was scanned for three times.
In each group, single-cell imaging was repeated for six cells, and each cell was scanned three times.
The dynamics of signalling networks that couple environmental conditions with cellular behaviour can now be characterised in exquisite detail using live single-cell imaging experiments.
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