Exact(2)
Both the Q matrix and K matrix were calculated using allelic data from 93 SSR markers (Additional file 1: Table S7) with single banding patterns [ 72] that were evenly distributed across all 19 rapeseed chromosomes.
Single banding pattern of PCR amplicons from several rows of a 384 well plate were visually confirmed by 1%% agarose gel electrophoresis and quantified with a Quant-iT™ PicoGreen dsDNA assay kit (Invitrogen).
Similar(58)
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