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In contrast, the heterologous expression of ScLac in E. coli achieved higher yields in a shorter cultivation time and required only a single affinity chromatography purification step.
The conversion of these three scores into a single affinity score can be interpreted as a multi-criteria evaluation, as presented by Meers et al. [46].
The protein has been purified using a single affinity step yielding >25 mg pure protein/liter culture and used as the coating antigen in anti-HCV EIA.
This indicated the presence of a single affinity site in the channel according to the model described by Schwarz et al., 2003 [25].
Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy.
To address this question, we created wild-type and ccr4-not mutant strains expressing Tap-tagged Rrp6 and immunopurified Rrp6 using a single affinity step.
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Not surprisingly, proteins that were reproducibly copurified with endogenous Oct4 tended to be more abundant in our single-affinity purification data set.
DOI: http://dx.doi.org/10.7554/eLife.00171.006 The mass spectrometry dataset from the cross-affinity purification fractions was quite different from that of the conventional single-affinity purifications.
Although there are some regions of double affinities between Arachis and the model legumes, most synteny blocks have a single main affinity and not two affinities interleaved (Figs. 2, and 3).
As such, an efficient single step affinity process to purify recombinant proteins from serum-containing medium was optimized.
[3H] A-369508 binds to a single, high affinity site on membranes containing the human dopamine D4.4 receptor.
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