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Since this cells expresses JIP1 is also possible that the interaction of TAK1 or MKK7 and VRK2A was mediated by endogenous JIP1, but in that case VRK2B also would interact with those MAP kinases, making these interactions specifics for VRK2A.

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Since this cell population can be easily identified through fluorescence microscopy, it may be an ideal source of ASCs for further experiments on stem cell biology and tissue engineering.

Since this cell assay demonstrated anti-inflammatory activity, in vivo anti-inflammatory activity assays were also performed.

We used wild H460 cells, since this cell line constitutively expressed abundant NPC2 protein.

We next determined ERCC6 mRNA expression in laser dissected RPE cells from the same eyes, since this cell type occupies a central position in AMD pathology.

Since this cell type is very sensitive to lysosomal inhibitors (data not shown), we avoided the use of such inhibitors in this experiment.

We chose N87 since this cell line shows detectable levels of both HER2 and HER3 and is known to be HER2-driven [35].

Our observation that some UP2-GFP+ populations express CK20 and GATA4/6 may also be evidence of superficial cell formation, since this cell type displays similar features in vivo [14].

Since this cell was a developing RGC and not isolated from adult tissue, the most likely explanation for this result is that this is a cell that was transitioning from an RPC to an RGC.

Since this cell line has previously been found to be resistant to apoptosis induced by TNF-family ligands, we utilized trichostatin A (TSA), a histone deacetylase inhibitor, to sensitize the cells to TNFα [30], [31].

Indeed, a rat extraembryonic endoderm cell line was found to express SSEA1 [18], but since SSEA1 is not a strong marker per se, and since this cell line also expressed parietal endoderm markers (as our XEN-P cell lines do), other identities were not considered.

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