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It was difficult to detect small molecules directly using conventional surface plasmon resonance (SPR) biosensors since the changes of refractive index, which was resulted by binding small molecules, were usually small.
It is essential, since the changes of the optical parameters (blue shift of the fundamental absorption edge, quantum states, luminescence), as well as of the conductivity and melting temperature (stability), are characteristic and usually examined in nanostructures.
Since the changes of the surface properties (chemical composition and wettability) during the sample relaxation lead to the stabilization of the sample properties (Figure 1) and, in this way, to the improvement of reproducibility of further experiments on biocompatibility, these were performed on relaxed samples only.
Since the changes of nucleo-cytoplasmic shuttling are controlled by the N-terminus of ERK1, we asked whether the deletion of this domain (E1Δ39), by providing ERK1 with a faster turnover would also convert ERK1 in a kinase functionally similar to ERK2.
The use of cross sectional design is the second limitation of this study, since the changes of QOL after proper management could not be evaluated.
Since the changes of EMG and hop distances were symmetrically but only approximately normally distributed, the score changes were statistically compared by application of the Wilcoxon rank sum test.
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