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As a strong oxidant, it cross-links lipids mainly by reacting with unsaturated carbon-carbon bonds, and thereby both fixes biological membranes in place in tissue samples and simultaneously stains them.
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However, these percentages were derived by staining different tissue sections for either basal or luminal markers and we did not detect any specific eLLRCs that co-stained for both lineages in sections when we simultaneously stained for both basal and luminal markers.
Cells were simultaneously stained on their surface for idiotypic and for isotypic Ig determinants using reagents labeled with different fluorochromes.
Cells were simultaneously stained with Annexin V-FITC (green) and PI (red).
Sections from all groups were simultaneously stained with hematoxylin and eosin (Park et al. 2005).
The single-cell suspensions from spleens were simultaneously stained with PE-conjugated anti-Ter119 Ab, FITC-conjugated Annexin V, and 7AAD to sort the apoptotic Ter119+ in spleens.
Negative control staining was performed by omitting the primary antibodies and, in order to avoid variability in the background staining due to the procedure, each treated animal was simultaneously stained with the corresponding control and processed at the same time.
To substantiate this observation, we assessed the cell death of Ter119+ cells by simultaneously staining the splenic cells with PE-conjugated anti-Ter119 Ab, FITC-conjugated Annexin V, and 7AAD [30].
For STAT5 phosphorylation, PBMCs were stimulated with IL-2 (10 IU/ml) or IL-7 (1 ng/ml) for 15 min and simultaneously stained with a monoclonal antibody against CD4-PacificBlue (OKT4, BioLegend).
Cells were simultaneously stained with propidium iodide to check for their viability.
Bone marrow cells from mice engrafted with human cells were simultaneously stained with anti-mouseCD45 and anti-humanCD45.
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