Males from these crosses were fixed for the X chromosomes of their fathers and used to simultaneously found two initially identical groups.
Both loci were initially simultaneously amplified with the general forward primer 5'-TGCTGTSAGCACTAATCTTGCG-3' and the general reverse primer 5'-TCGAACCCGGGAGAGGTAGACCA-3', following the protocol of Wright et al. [ 23].
All considered exposure terms were initially entered simultaneously and manual, backward selection criteria were applied to sequentially eliminate exposures that were not independently associated with symptom severity score.
The temporal similarity of viral detection in nasal cavity, submandibular lymph nodes, tonsils, spleen, and liver, within the WA MXPV infected animals, supports the supposition that virus initially spread simultaneously through lymphatics (lymph nodes and tonsils) and blood stream (spleen and liver).
Initially, SI increases simultaneously with the F− concentration, until F− concentration reached a value of 6 due to long residence time of water rock interaction which lead to the release of F− from fluorine-bearing minerals (Rafique et al. 2015; Li et al. 2015).
The ability of T. thermophilus to produce simultaneously PHAs and RLs was initially tested in MSM containing either glucose (2% w/v) or sodium gluconate (1.5% w/v) as sole carbon source while the initial phosphate concentration was adjusted at approximately 36 mM (see Material and Methods section).
By using a state-of-the-art setup designed specifically for the in situ study of both DS and SWAXS simultaneously (SWD), the crystallization of initially amorphous PBI has been followed in real time from both the structural and dynamics points of view.
Orexins (also named hypocretins) are neuropeptides initially discovered almost simultaneously by de Lecea [ 1] and Sakurai [ 2] and are identified as neurotransmitters in lateral hypothalamus (LH) neurons.
In addition to normalized counts, we also consider mean-based filters for the RPKM (Mortazavi et al., 2008) measure, which was initially proposed to simultaneously normalize RNA-seq data for biases due to library size and gene length.
Despite the timing, U.S. officials insisted that the two big breakthroughs on Saturday — on the nuclear deal and on the detainees — were not initially designed to conclude simultaneously.
