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A 50-ng DNA sample was simultaneously fragmented using Illumina Enrichment Sample Prep and tagged with adaptors.
Individual Nextera XT libraries were simultaneously fragmented and barcoded using the 96 sample Nextera Index kit (Illumina, San Diego, California, USA).
By allowing co-eluted peptides to be simultaneously fragmented, one increases the information collected per spectrum, albeit at the expense of introducing higher level of noise, and thus one may improve protein identification rate as well as the confidence in protein identification and quantification.
Another approach to exploit data-independent acquisitions involves using an additional separation technique prior to fragmentation to increase measurement sensitivity and the ability to associate simultaneously fragmented precursor ions with their corresponding fragment ions.
Briefly, the input DNA (50 ng for each pool) was simultaneously fragmented and tagged with the adapter sequences by transposome in a single reaction step.
The genomic DNA sample was simultaneously fragmented and tagged with a biotin containing mate pair junction adapter, which left a short sequence gap in the tagmented DNA.
Similar(52)
Urban sprawl increases the connectivity among urban habitats while simultaneously fragmenting non-urban habitats such as forests and grasslands.
This paper presents an approach which simultaneously fragments data vertically and allocates the fragments to appropriate sites across the network.
The sonic waves radiate out on multiple levels simultaneously, fragmenting the intrinsic connections within the warrior, his body, brain, mind, and soul, fragmenting his family, social supports, relationship with his community, the organizations charged with his care, the institutions and leaders responsible for protecting the country, and the entire culture.
The sonic waves radiate out on multiple levels simultaneously, fragmenting the intrinsic connections within the service member (mind, body and soul), his/her family, social supports, his relationships with the community, the organizations charged with her care, the institutions responsible for protecting the safety of the country, and the entire culture.
All 96 PCR amplicons were subjected to transposase-based library construction using the Nextera kit, which simultaneously fragments the DNA and adds adaptors needed for multiplex sequencing.
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