Sentence examples for simultaneously fixed from inspiring English sources

After 12 or 24 h of culture the generative cells or sperm cells in the pollen tubes were isolated and simultaneously fixed for 30 min in fixative containing 4%% (w/v) paraformaldehyde, 0.1%% (v/v) glutaraldehyde and 2%% (v/v) polyoxyethylene (20) sorbitan monolaurate in microtubule-stabilizing buffer.

If max < 1 / 2, then there are z 1 ∈ A and z 2 ∈ B such that z 1 ∈ ( T 2 z 1 ∩ T z 2 ), z 2 ∈ ( T 2 z 2 ∩ T z 1 ), i.e., z 1 and z 2 are, respectively, best proximity points of T : A ∪ B → A ∪ B in A and B, respectively, and simultaneously, fixed points of T 2 : A ∪ B → A ∪ B, respectively.

Results are presented from day 4. Clonogenic survival was assayed as follows: 500 cells (parental E006AA, E006AA LV-non-silencing-shRNA, and E006AA LV-AR-shRNA) were plated per well in 6-well plates, allowed to adhere and colonize for six days, then simultaneously fixed and stained in a 0.5% crystal violet/25% methanol solution and colonies were counted manually.

To detect apoptotic cells we incubated the preparations with FITC-annexin V, rinsed in washing buffer (10 mM HEPES/NaOH, pH 7.4; 140 mM NaCl; 5 mM CaCl2), and simultaneously fixed and permeabilized in 1% paraformaldehyde containing 0.25% Triton X-100/PBS at room temperature for 20 min. Samples were then immunolabelled with anti Rad51 or anti pS2056DNA-PKcs as previously described.

In the present work, we describe a new immunostaining protocol in which spheroids are simultaneously fixed and permeabilized in a mixture of paraformaldehyde and Triton X100 and then gradually dehydrated in methanol before incubation with labelling antibodies.

Cells were stained with PE-Cy5.5-anti-CD4 and APC-anti-CD25, then cells were simultaneously fixed and permeabilized with Fix/Perm Buffer (eBioscience), and intracellular staining with PE-anti-FOXP3 mAb was developed.

The country is not in the best shape to simultaneously fix a sinking economy, a withered government and an ailing planet.

We consider optimization problems in fixing the orientations of the cells and simultaneously fixing the directions of the cuts that are specified by a given slicing tree; the goal is to minimize the area of the chip.

The computational algorithm used in PhotoModeler, called bundle adjustment, essentially applies the colliearity equations (Eqs. 1 and 2) to simultaneously fix (1) camera orientations, (2) object and image point coordinates and (3) the residual error, with an objective to minimize the residual error.

This could not only decrease unemployment and increase productivity, but simultaneously fix the situation we have right now, where it's possible for the unemployed to actually earn more in equivalent benefits than the cash incomes of those who are employed.

Solutions containing 1% crystal violet (Merck KGaA, Darmstadt, Germany) with 70% ethanol (Wako, Osaka, Japan) were used to simultaneously fix and stain surviving cells to make them easily visible.