Near-membrane fluorescence signals track the gating of N-type Ca2+ channels with a kinetic resolution of about 10 ms, provide a simultaneous and independent readout from several channels, and allow their locations to be mapped with sub-micrometer spatial resolution.
For cell-based assays, an exemplar application reading out protein aggregation using FLIM FRET is presented, and the potential for multiple simultaneous FLIM (FRET) readouts in microscopy is illustrated.
Key to these results is the use of different pairs of 9Be+ hyperfine states for robust qubit storage, readout, and gates, and simultaneous trapping of 24Mg+ "re-cooling" ions along with the qubit ions.
This method uses signal analysis techniques, for example waveform fitting, to measure the charge collected by each readout channel, from several, almost simultaneous events.
The first simultaneous PET/MR (Siemens mMR) was based on APD readout and therefore not capable of TOF [45].
In this paper, a miniaturized sensor patch (MSP) with simultaneous impedance and temperature measurements and fully integrated readout is cleverly designed.
The goal of the EU-FP7 Hyperimage and Sublima project (http://cordis.europa.eu/project/rcn/87568_en.html) is to develop a simultaneous whole-body PET-MR system based on a completely different readout scheme with MR-compatible SiPMs and electronics.
A PIA with a full complement of 4 readout circuits would span 1 cm and could acquire simultaneous data from selected regions of 1024 electrodes at sampling rates up to 9.3 kHz.
In addition to matching the detection profile of the standard ELISA, the nanocoaxial array provides a simple electrochemical readout and a miniaturized platform with multiplexing capabilities for the simultaneous detection of multiple biomarkers, giving the nanocoax a desirable advantage over the standard method towards POC applications.
The use of spiral-based readout gradients effectively reduces the minimum scan time required due to simultaneous data acquisition in three k-space dimensions (kx, ky and kf2).
