Here, we present the results of the simultaneous amplification of these genes in strains with different genetic backgrounds.
A single pair of degenerate primers was designed from these sequences to allow the simultaneous amplification of parts of the movement protein genes of ArMV and GFLV.
Simultaneous amplification of 482 and 359 bp fragments of thenuc and ail genes was obtained using the primer pairs in a single reaction.
The assay utilises simultaneous amplification of target RNA and a recombinant RNA competitor in a single reaction, using the same pair of primers.
However, the simultaneous amplification of IS6110 and MPB64 in patients with osteoarticular tuberculosis (TB) and IS6110, MPB64, and protein b in patients with TB meningitis (TBM) resulted in enhanced sensitivity of 82.5% and 86.63%, respectively [12, 13].
In contrast, the simultaneous amplification of the activities of the two enzymes yielded more than 250% increase of the lysine specific productivity in lactate minimal medium without affecting the growth rate or final cell density of the culture.
However, the use of microarray-based DNA methylation analysis has been limited by the low throughput of sample preparation due to the difficulty in simultaneous amplification of multiple targets.
These kits allow for the simultaneous amplification of the CODIS core loci and the European Standard Set loci, as well as a few Y-STR loci in addition to the standard sex-determining marker Amelogenin.
This Y STR 20plex, which utilizes 34 different PCR primers, is the first to include a simultaneous amplification of all the markers within the European "minimal" and "extended" haplotypes.
Similarly, a second set of three primers were designed for simultaneous amplification of 540 bp and 655 bp fragments specific to the coat protein gene of EACMCV and ACMV, respectively.
However, the simultaneous amplification of all the SNPs excludes this possibility because only single alleles have been observed and all other sites tested are consistent.
