Suggestions(1)
Exact(6)
Protein expression vectors designed for large-scale protein production often include one or more specific tags to such transcripts, to simplify the purification of the targeted protein.
Heat treatment is often used during the processing of plant-derived biomass to precipitate host cell proteins (HCPs) and thus simplify the purification of target proteins.
Because we expected the discharge sources to be rather messy and possibly not very selective, we decided to use two guides which would simplify the purification of the beams prior to the collision studies.
Although these self-cleaving systems simplify the purification of well-expressed proteins, the large size of the intein-CBD fusion tag can decrease target protein solubility [2], while sortase-His6 fusion tags do not increase target protein solubility [9].
This will undoubtedly simplify the purification of this enzyme.
At this point in the sequence, the base had to be removed to prevent salt precipitation during the ozonolysis stage, and to simplify the purification of the final product.
Similar(54)
Heat treatment can simplify the purification protocol of thermotolerant proteins as well as remove any soluble aggregate.
As a result of recloning cgAUS1 proenzyme into pGEX-6P-1 the recently developed method could be advanced to successfully produce large amounts of pure, active and soluble cgAUS1 wild type (58 kDa + 26 kDa GST tag) in E. coli and simplify the purification process by a reduction of the four ion exchange purification steps to only one affinity chromatography step [5].
The secretion capacity of B. choshinensis was expected to simplify the purification steps to obtain rTFF1 more easily.
One of the advantages of the P. pastoris expression system was that the purities of expressed recombinant proteins were very high according to the description of the Multi-Copy Pichia Expression Kit, which could greatly simplify the purification procedures.
● Capping: acylation of the unreacted 5′-OH ON terminal groups by acetic anhydride in pyridine and tetrahydrofuran to minimize deletion products and simplify the purification process.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com