However, a simplified procedure consisting in a single step of fixation/permeabilization in cold methanol or methanol/acetone without prior aldehydic fixation led to a massive delocalization of membrane proteins (data not shown).
The method is compared to a simplified procedure suggested in the literature where stress singularities associated with corners at kinking or branching are neglected in the representation for the dipole density distribution.
The legal documents and the Code can be amended through the simplified procedure set out in Article XV of the Convention.
A simplified procedure is firstly proposed in order to identify the organic fluid that guarantees the highest performance under design conditions.
Although this lessened the participants' burden, this simplified procedure may have allowed bias in the diagnosis of ADHD in adults.
Equation is developed and described to demonstrate the basic behavior, leading to the development of simplified procedures used in various design loads, specially ACI.
Other PLA2s have also been purified using simplified procedures based in CM-Sepharose and/or reverse phase, as for example, venom PLA2s from Bothrops moojeni [ 21, 32, 33], B. pirajai [ 34, 35], B. jararacussu [ 36, 37], B. alternatus [ 8, 38], Cerastes cerastes [ 39], and Elaphe climacophora [ 40].
The order of design equations in the simplified procedure is reduced to that of the undelayed system part.
These results, obtained at ambient temperature, were then incorporated in a simplified procedure that is proposed for the design of fire protection systems of CFRP-strengthened RC flexural members.
It is shown that the accuracy in the end result may in practice be maintained by this simplified procedure.
