Conservation of energy and simplification of protocol are important design considerations in this scheme.
Since no significant matrix effect has been found for any of the water types analyzed, quantification could be carried out by using conventional external calibration, thereby allowing a high analytical throughput and a valuable protocol simplification.
For protocol simplification, both arterial and venous TBR, in the time interval between 40 and 60 min post injection, could be used; however, arterial and venous TBR values should not be interchanged as venous values underestimate arterial values.
This simplification shortened the protocol and made the method cheaper, important for high level multiplexing.
Its advantages are a simple application, provision of parametric estimates that characterize the response, simplification of the protocol, economization of experimental effort, and facilitation of rigorous comparisons among the effects of different compounds and experimental approaches.
Further simplification of the protocol into a five-species SnM-PCR should be feasible as preliminary data have shown that the P. knowlesi-specific primer was compatible in silico with the other four human Plasmodium-specific primers.
In 2012 13, the need for "the most user-friendly guideline" was highlighted, particularly in terms of monitoring, and the simplification of the protocol was recognised as a facilitator: "There were the two administration regimens… It is a lot clearer now… It's easier when there is just one protocol and everyone sticks to it" (TO2-2012/13).
In addition, for simplification of future protocols, we also investigated simplified uptake parameters, i.e., standardized uptake values (SUVs) and tumor-to-blood ratio (TBR).
This was again important for simplification of future protocols as arterial cannulation could be omitted if venous sampling was proven to be a valid substitute.
The initial success prompted us to evaluate, with the help of the AI analysis, further protocol simplifications.
