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With the identification of novel targets is should be feasible to create simple point of care assays aimed at these specific targets.
Among these, simple, point of care, inexpensive tests which require only a drop of blood from finger-prick and give reliable results within minutes are a must for expansion of testing services and for reaching mobile and marginalised populations.
The POCT that will be evaluated is called Flexicult™ and is a simple point of care culture procedure, and provides clinicians, at the point of care, within 24 hours, with a microbiological diagnosis of bacterial UTI by quantifying bacteria in the urine sample (or not) and determining sensitivities of any identified pathogen to the antibiotics most commonly used for UTI in primary care [ 8].
This study represents an early stage in the investigation of QSSMs as novel biomarkers for P. aeruginosa and to bring this test to routine clinical practice would require further validation steps and the development of simple point of care assay, similar to those used in diabetic control monitoring.
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The Royal Tropical Institute in the Netherlands has developed a rapid and simple point-of-care test, the Brucella IgM/IgG immunochromatographic lateral flow assay for the serodiagnosis of human brucellosis [6], [7].
Although simple point-of-care assays are available and routinely used for diagnosis of HIV infection and malaria [ 6], this is not the case for TB.
Simple point-of-care tests such as the LFI may become available in the near future and would enable rapid identification of isolates and direct detection in clinical specimens.
The development of a rapid, simple, accurate point-of-care techniques for oral microbiota profiling, particularly the detection and quantification of oral pathogens, has thus become a matter of urgency.
To utilize lay counsellors to perform simple, rapid point-of-care tests for both syphilis and HIV could prove a concrete and feasible opportunity to reduce the disease burden of these two infections.
The simple, model point-of-care system presented here, which can be easily applied in every dental office, reduces both time and costs for diagnostics and eliminates the necessity for transport to external laboratories and therefore facilitates crucial fast treatments in the future.
A simple, low-cost, point-of care version of this assay has been shown to have considerable utility when screening for TB prior to antiretroviral therapy [ 22].
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