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In general, simple BLAST or advanced PSI-BLAST (Position Specific Iterated BLAST, [18]) is used as a first choice for functional annotation of predicted proteins or proteins of unknown function.
As expected, profiles generated from three PSI-BLAST (12) iterations provide better coverage in almost all families when compared to a simple BLAST search.
The missile's warhead is made with a mixture of chemicals rather than a simple blast mechanism.
For oil and gas facilities offshore, and often onshore, the computational fluid dynamics (CFD) tool FLACS is usually applied, while others use simple blast curve formulations, like the TNO-Multi Energy Method.
Newt's simple, blast 'em all strategy, fits brilliantly into their tough guy ego.
The first approach is to identify genes based upon homology with genes that are already publicly available in sequence databases, usually by simple BLAST searches.
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For example, the extreme case of E. coli is probably due to the simple-minded BLAST reciprocal pairwise comparison that was used in [ 4] to identify genes belonging to the same gene family.
For example, within the MIKC MADS-box gene family, phylogenies uncover greater levels of overlap among our EST sets than were inferred from simple BLAST-based analyses.
This is one key advantage of my model-based tests over simple BLAST-type analyses that only look at gross sequence similarity.
CNetA was compared with a seed-and-extend method MI-GRAAL [ 14], and two simple BLAST-based methods [ 16] as well as CNetQ.
We also provide a pipeline for validation of the array in other species using a simple BLAST-based sequence identify filter.
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