Cells were sandwiched between thin layers of agarose on a microscope slide, lysed at neutral pH, electrophoresed, and stained with a silver dye.
Concerning the success in their identification, it is interesting to mention that more proteins were identified from gels stained with Coomassie, although the number of protein changes detected using silver dye was higher (Table 2).
The bands were dyed with silver nitrate, and the isoelectric point was evaluated using the analytical unit of FastSystem employing a broad range standard kit (pH 3 10) (Amersham Biosciences, Buckinghamshire, United Kingdom) as a protein calibrator.
He then reflected on the function of the pancreas and the pancreatic duct, asking whether it is a type of a vein or artery, and simultaneously answering in the negative by stating that he has never observed blood in the pancreatic duct, while the fluid present in the duct dyed the silver probe similarly to bile [ 2, 5].
He wore vintage shirts in wild geometric patterns; his hair was dyed orange or silver one week, shaved off the next.
She met the camera head-on, dressed in hand-loomed textile dyed indigo, a silver earring half-hidden in the cascading curtain of her dark hair.
PCR products were loaded on 2.5% agarose gel and 6% poly-acrylamide gel, stained with silver nitrate dyes and visualized under UV illumination.
Samples (24 μL with 6 μL of 5× loading buffer) were boiled and loaded on a polyacrylamide gel and run at 200 V. Polyacrylamide gels were stained with silver stain dye.
In fact, co-immunoprecipitates display similar intensity of bands with both Ponceau S and silver staining dyes and a comparable number of PARP1 and TIMELESS peptides by mass spectrometry.
We noticed that when we immunoprecipitated tagged TIMELESS after its expression in human cells, by staining with either Ponceau S or silver staining dyes, we consistently detected a second, faster migrating band that co-precipitated at an apparent, near-stoichiometric ratio with TIMELESS.
Ms. Getzendanner, 58, had a great grin, and silver-gray hair that she never dyed.
