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Silk was sampled from webs within 24 hours after spinning.
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Dragline silk samples were taken from the radial threads of the orb web while flagelliform silk was collected from the sticky spiral of the web [21], [22], [23], [24].
For dragline silk collection, the silk was pulled out of the major ampullate gland with forceps.
Because spider silk is prone to strong auto-fluorescence signals, Alexa 488 goat anti-rat (Invitrogen) was added to the samples to mark the spider silk fibres by unspecific attachment.
Silk is also verboten.
Some pieces of silk were left plain.
Spider silk is a good start.
Valerie's agonizing over having picked silk ("cutting silk is hard, finishing silk is really hard").
No furs, plumes or silk are worn.
Bombyx mori silk is the most popular silkworm silk.
The analytical markers monitoring the structure and properties of silk were statistically integrated using exploratory principal component analysis (PCA), to obtain correlations between the effect of the different artificial ageing environments and the properties of the historic silk samples.
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