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Silk gland FAIRE, from top to bottom: silk gland FAIRE peaks, BmE FAIRE peaks, and Ubx chip peaks47.
Two silk gland-specific OCRs were subsequently identified in the silk gland Sericin 1 gene.
Additionally, we compared the motifs detected in the OCRs of the silk gland and BmE cells (Fig. S2, Table S3) and identified seven silk gland-specific motifs.
These features make the silk gland a good model for studying transcriptional regulation networks.
Pink peaks are silk gland FAIRE-seq and RNA-seq peaks.
Hijirida, D. H. et al. 13C NMR of Nephila clavipes major ampullate silk gland.
Orange bar represents silk gland OCRs; blue bar represents BmE OCRs.
"There's no lab anywhere in the world where somebody has an artificial silk gland".
While binding motifs for SGF-1 were detected in an OCR 6-kb upstream of the Fibrohexamerin gene in both the silk gland and BmE cells, an Awh-binding motif was detected only in the OCR of the silk gland.
These OCRs in the promoter region combined with the two OCRs in the gene might therefore constitute a silk gland-specific CORE that can promote Sericin 1 expression within the silk gland.
Notably, however, we detected higher levels of Sericin 1 expression in the silk gland than in BmE cells.
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