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Nucleic acids were extracted using silica extraction technology (NucliSENS® easyMAG® automated technology bioMérieux, Craponne, France).
DNA was extracted using an abbreviated silica extraction protocol.
As droplets exit the Teflon tubing, aqueous samples are extracted into the hydrophilic fused silica extraction capillary while the oil phase continues toward the outlet of the hydrophobic PDMS device.
Genomic RNA was extracted from the stool samples using a Nuclisens Easymag magnetic silica extraction method (Biomerieux, France).
This white RHA was used for silica extraction and preparation of nanosilica.
An acid washing step was used to remove the small quantities of minerals prior to silica extraction from RHA in the following manner.
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Silica extractions were performed using QIAamp DNA micro extraction kits (Qiagen) following the manufacturer's guidelines, although using double the suggested volumes of all reagents prior to the wash stages (proteinase k, buffers AL and ATL, 100% Ethanol).
Although we assayed various alternating extraction methods such as phenol/chloroform followed by the GuSCN-silica protocol and vice versa, the efficiency was similar to the currently used two consecutive GuSCN-silica extractions.
Freeze drying samples was the best preservation method when samples were extracted using the silica based extraction method peqGOLD™.
[H4]‐20‐HETE (10 ng) was added, and the sample was extracted with acidified CHCl3/CH3OH (2:1) and purified by silica solid‐phase extraction.
The EETs and DHETs were extracted from the tissue homogenates with acidified CHCl3/CH3OH (2:1) and purified by silica solid‐phase extraction, separating EETs and DHETs.
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