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Separation was done on silica column derivatized with sulfoalkylbetaine groups (ZIC-HILIC).
Fatty acid methyl ester was extracted by hexane and analyzed through a GC-2010 GCMsystemem (Shimadzu, Japan) equipped with a HP-88 fused silica column (Agilent, USA).
Acetyl-TAG could be separated from long-chain TAG by silica column or by reverse phase chromatography.
The reversed phase chromatography utilizes a monolithic C-18 modified silica column, which produces fast and efficient analyses.
In recent scenario, the condition of monolithic silica column is very auspicious.
Chen et al. prepared C18 monolithic silica column by using ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate ([bmin]BF4)).
The silica column was then eluted with n-hexane (100 mL) to remove the residual non-active apolar components.
Fig. 1 Macropore in a monolithic column (Cabrera, 2004) Fig. 2 Back pressure of conventional and monolithic silica column.
The column consisted of a phenyl-bound silica column (100 × 4.6 mm; 5 µm particle size) from Thermo Scientific.
When passed through a silica column to further purify the factor, it partitions into the glyco- and polar lipid fractions.
However, under the conditions of the kit, the silica column will bind to both DNA and RNA.
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