Sentence examples for silent restriction from inspiring English sources

Thirteen unique silent restriction sites were introduced in the pol gene encoding HIV-1 RT, allowing easy introduction of mutations.

The B. pumilus lipase gene was synthesised to contain 13 unique silent restriction sites within the ORF, and placed under the regulation of T7 promoter of the pET expression system.

In addition a PstI silent restriction site was incorporated into the mutant primers for candidate screening.

A silent restriction site was incorporated in each to assist rapid identification.

A silent restriction site was incorporated into each primer to assist rapid identification.

The PCR fragments were assembled using the unique silent restriction sites at each end of the fragments.

For convenience, the MblCK202I primer also introduced a silent PstI restriction site that facilitated recognition of mutated construct.

Two PCR reactions using two mutagenic primers 6Lstop (5'-CTTTCCAATTAAAACAGCTCGG-3') and 6Rstop (5'-CCGAGCTGTTTTAATTGGAAAG-3') that introduced a stop codon at Y755 and a silent Mse1 restriction site for selection and two outside primers (5R: 5'-GATCTGCACCGGGTAGAAGTTG-3' & 5L: 5'-CAACCAGGATGTGGTGGAGATG-3').

The S892A mutation in exon 19 was introduced into the 3' arm along with a diagnostic silent NheI restriction site.

Although SMS messaging is silent, the restriction on structure means that it may be difficult to carry on a potentially complex real-time interaction between patient and provider.

The click-linked primers were also designed to introduce a silent BamHI restriction site into the region encoding the MYG fluorophore (by altering the glycine codon from GGC to GGT).