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As expected from the positioning of the MYC2 silencing region in non-translated 3' UTR of the gene, we found no significant reduction in the accumulation of the MYC2-like transcripts in MYC2-VIGS plants compared to EV control plants, indicating that VIGS silencing was confined to MYC2 TF (Additional file 1: Figures S1 and S2).
Primers for CHS mRNA quantification (f 5'-TGCTCTGAGATCACAGCCGTTA -3', r 5'-CCGACGAGGGAGTCAAGGT-3') were designed outside the silencing region, using the Primer Express software (Applied Biosystems).
Exon 12 also contains a silencing region that includes GGG triplets.
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The non-transcribed spacer region from the rDNA locus, NTS1-2, walsolso mildly upregulated (1.8-fold), although the significance of this is difficult to assess as the overall expression of this feature is low, as predicted based on its location in a transcriptionally silenced region [60].
We used the Hermes transposon mutagenesis system to generate mutants [ 37] (Fig EV1A) and isolated a clone that was both 5FOA-resistant and red on low-adenine (YE) plates; thus, this mutant had an extended silenced region surrounding the matK region (Fig 1B).
EBV selectively methylates and silences regions of its genome which would, in the infected host, be detrimental to viral persistence, whereas other viral genes, which are critical for propagation of viral DNA, are expressed from alternative promoters which are protected from the host cell methylation machinery [52].
These domains mark contiguous regions sharing an epigenomic property, such as actively transcribed or poised genes, or heterochromatically silenced regions.
Simultaneously, loss of methylation in otherwise silenced regions takes place, a process named "global hypomethylation," with increased overall gene expression level due to weakened transcriptional repression.
Cellular senescence, an important component of mammalian aging, has recently been associated with decreased heterochromatinization of normally silenced regions of the genome.
The tracking accuracy is then further improved by the inclusion of a silence region detection step and estimation of the noise-only covariance matrix.
The averaged SNR value was estimated as the ratio of the averaged frame energy to the averaged noise energy of the initial silence region in each test utterance.
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