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Antisense antimicrobial therapeutics are synthetic oligomers that silence expression of specific genes.
Coupling of dCas9 to a transcriptional repressor domain can robustly silence expression of multiple endogenous genes.
We have designed a system to silence expression from nontransposed sequences, making it possible to determine more specifically the amount of expression resulting from transposition.
We proposed to deliver microRNAs (miRNAs) to silence expression of the TGF-β1 gene and to investigate the effectiveness of miRNAs in down-regulation of the TGF-β1 gene in vitro and in vivo.
In addition, CRISPRi can be used to effectively, specifically, and homogeneously silence expression of up to 2-3 genesimultaneouslyly, which also allows us to test for epistasis among target genes.
Furthermore, we discovered that directly converted cells fail to adequately silence expression programs of the starting population and that the establishment of unintended GRNs is common to virtually every cellular engineering paradigm.
Similar(19)
We then silenced expression of both Nrp genes with shRNA-expressing lentiviruses.
A single copy of the transgene effectively and uniformly silences expression of a target gene (white) in transgenic flies.
These experiments demonstrate that SECs are an excellent screening tool to identify siRNA sequences effective in silencing expression of genes of interest.
RNA interference (RNAi) therapy, which silences expression of specific target genes, represents a promising approach for treating viral infections in mammalian hosts.
We infer from these findings that one mechanism of impairing P. rapae hemocytic immune reactions is by silencing expression of Pr-SR.
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