In contrast to Kf-exo–, pol β exhibited significantly less binary binding affinity to the modified templates.
We believe the difference is because the cell-binding assay is significantly less sensitive than the affinity chromatography assay and is largely reporting on the higher affinity interaction between cyclopamine and the 7 TM site (which cannot be competed with oxysterols).
We also investigated if glycosylations could have a potential influence on the likelihood of peptides being presented on MHC class II molecules, and demonstrated that the MHC class II ligands are significantly less glycosylated compared to affinity matched non-ligands.
Using the state-of-the-art MHC class II binding predictions, NetMHCIIpan, combined with prediction of local protein structure by NetSurfP, our analysis revealed that HLA class II ligands are significantly more exposed and have significantly less local secondary structure elements compared to affinity matched non-ligand peptides within the same source protein.
The dansyl equivalent of ABEA-X-BY630, AUEA-dansyl (10), did show measurable affinity for the A1 receptor, but was significantly less potent (Table 2).
Similar experiments exploring the effects of defects on hybridization affinity, also on microarrays, indicated that hybridization is significantly less error-sensitive.
mAbs that bound BoNT/A2 and BoNT/A3 poorly (KD>200 nM, C25, AR1, AR2, and AR4) showed significantly less extraction than clonally related mAbs binding the same epitope with affinities less than 100 pM (CR1 and CR2).
Nevertheless, our consistent observation that ethanol titers were significantly higher than 1-propanol implies that propionyl-CoA or propionaldehyde might have less affinity towards ADHs than acetyl-CoA or acetaldehyde.
For the SwH-NOX F17A and E20K mutants, we observed significantly less quenching of the SwHaCE signal than for the WT, suggesting a reduced binding affinity for each of these mutants for SwHaCE.
The affinity of carbofuran for CYP enzymes involved in the oxidation to 3-hydroxycarbofuran is significantly less in HLM (Km = 1.950 mM) than in RLM (Km = 0.210 mM), or MLM (Km = 0.550 mM).
