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Strains deleted for one rpf gene only showed no defects in their ability to resuscitate however, the triple mutants were significantly defective for resuscitation in this model.
Significantly, defective and exposed edges in the carbon nanostructure owing to a higher percentage of N-doping are formed, which is also supposed to offer effectively reactive sites for the ORR [15].
The pollen grains in pksa or pksb single mutants are fertile but has abnormal pollen exine; however, their double mutants produced sterile pollen due to the significantly defective pollen exine (Dobritsa et al. 2010; Kim et al. 2010).
In contrast, the UTI89 ΔhofQ mutant was significantly defective in colonizing the kidneys on day 7 post-infection (p = 0.03, Fig. 3B).
Our results showed that the C. jejuni Δppk2 mutant was significantly defective in poly P-dependent GTP generation, indicating that C. jejuni PPK2 catalyzes poly P-driven GTP synthesis (Figure 1A) similar to PA0141 and rv3232c.
The C. jejuni Δppk2 mutant was significantly defective in invasion (Figure 7A) and intracellular survival (Figure 7B) within human intestinal epithelial cells, suggesting an important role for PPK2 in C. jejuni pathogenesis.
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Therefore, we assessed the ability of C. jejuni Δppk2 mutant to generate GTP from poly P. The C. jejuni Δppk2 mutant was significantly (P≤0.05) defective in poly P-dependent GTP generation compared to WT (Figure 1A), while complementation restored the ability of the Δppk2 mutant to generate GTP to levels comparable to WT (Figure 1A).
However, significantly fewer defective calves than expected were found (χ2 = 11.45, p < 0.001).
Significantly positive defective DNA repair-gene expression was associated with an abundance of DNA damage (53BP1 foci).
Importantly, expression of wild-type Pol κ in siRNA-mediated Pol κ-depleted cells significantly rescued defective Chk1 phosphorylation.
In Rpl11-deficient zebrafish, the vast majority of iron and heme metabolism-related genes were downregulated, contributing significantly to defective hemoglobinization and hematopoiesis.
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