In our study, 13,806 assembled gene models (73.07% of the total assembled contigs, Figure 3A) were considered high confidence annotations due to the presence of a significant high stringency BLAST hit in other angiosperm species, as well as a predicted CDS.
No significant difference in stringency was found between male and female examiners or age of examiners.
P ≤ 0.01 was considered significant, with a stringency of ± 1.5-fold difference in expression levels.
Five genes (C12orf35, DYNLT1, IRS2, SKIV2L2, and TARDBP) were significant at high stringency (qSAM = 0%, pLIMMA < 0.001, qLIMMA < 5%).
There was no significant correlation of stringency with examiner year of birth (r = -.028, n = 1162, p =.336).
Previous studies have shown significant improvement in selection stringency by using GS-knockout CHO cells, which eliminate background GS expression from the endogenous GS gene in CHOK1SV cells.
On the one hand, a number of studies have found no statistically significant effects of the stringency of labour legislation on employment and unemployment rates.
At the same level of statistical stringency, the significant gene lists derived from four different comparisons for either TNT or RDX shared very few common genes (Fig. 2), suggesting different genes may be significantly altered under different conditions.
Indeed, both RNA silencing methods indicate significant differences in gene stringency and reveal acpP as the most stringently required essential gene, followed by ftsZ, out of the four that were studied.
With medium stringency, no significant cluster was presented.
