For Figure 6C,D, we applied the Bayesian decoding algorithm to these significant sequences in order to calculate the path length, ahead length, and behind length.
Although this will affect the number of significant sequences found in the searches by a factor of two or more [41], pyrosequencing yielded orders of magnitude more sequence per dollar than comparable Sanger sequencing, more than compensating for these missing sequences.
In the present study, the significant sequence effect in the analysis of Cmax was considered a random event, given the single-dose design, involvement of healthy volunteers, sufficiently long washout period, predose levels from the second period being < 1% of the corresponding Cmax, validated assay procedure, and diazoxide being an exogenous entity.
In spite of the significant sequence bias in the USE region, no upstream region was beneficial to search accuracy.
Although there appears to be some significant sequence dependence in the data for FSFG-K in-buffer (green, right), the S/N in the FG-N case obscures whether this is present there also.
Sequence insertions (for TBX-33), changes in the variable region (for TBX-39/40 TBX-39/40nificand significantangesequence key 310changesor TBX- 39/40) are hinhlighthe in blue frames.
An amino acid alignment revealed significant sequence conservation in the C-terminal third of the protein, whereas the N-terminus showed less conservation.
Besides, analysis of a multiple sequence alignment among Henipavirus, Morbillivirus and Respirovirus N proteins (see supplementary Fig. S1) revealed a significant sequence divergence in the NTAIL region, in agreement with earlier observations pointing out a higher sequence variability in disordered regions as compared to structured ones [38].
Previous studies have shown that mouse, rat and hamster rDNA repeats share significant sequence similarity in the spacer promoter region of the IGS [ 27].
Five of the 6 myosins exhibit significant sequence identity in the so-called "dilute" domain.
