Exact(7)
The optimum OD was 0.4 manifested by significant GUS expression.
Among the various concentrations tested 100 μM AS resulted in significant GUS expression.
In embryos, significant GUS staining was detected in the vascular tissues and scutellum cells of embryos (Fig. 8).
Exposure of the explants to 0.4 OD Agrobacterium for 10 min was found to be optimum as significant GUS staining was observed whereas 15, 20 and 25 min of infiltration besides resulting in GUS signals lead to contamination (Figure 2C).
Interestingly, eight lines out of fifteen showed a low but significant GUS transcript accumulation compared to the full length minimal promoter.
In accordance with the results obtained from the histochemical analysis, qRT-PCR experiments did not detect significant GUS transcripts accumulation in lines carrying the -205:: GUS fusion.
Similar(53)
Even though not statistically significant, mean GUS activity values were also higher in both P-miR164a::GUS lines after TMV-Cg infection.
However, significant level of GUS expression was observed at 100 μM AS after which GUS expression combined with necrosis was observed (Figure 2D).
We did not find a significant difference in GUS activity among Oshsp17.3p5, Oshsp17.3p11, Oshsp17.3p5/athsfA5, Oshsp17.3p11/athsfA5, Oshsp17.3p5/athsfB2a and Oshsp17.3p11/athsfB2a plants.
These tests showed that the waterlogging treatment induced significant increases in GUS and ADH enzyme activities, while the control and clinostat treatments showed no response.
Of note, AtHSFB2a is highly AZC- and HS-inducible, but we did not find a significant reduction in GUS activity with AtHsfB2a knockout during AZC treatment.
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