These cells showed a significant defect in DNA ligation, which was rescued by addition of recombinant FUS or by correcting the genomic FUS mutations.
Importantly, a marked decrease in the capacity of AMA1 to co-immunopurify RON2 was observed in the RON2IL/AA compared with RON2WT (Fig. 3c) that correlated with a significant defect in invasion (Fig. 3d).
C Feeding suppression induced by 1% 1-octanol shows a significant defect in ser-2 or tyra-2 mutants.
ATG5-/ progenitors exhibited a significant defect in B cell development at the pro- to pre-B cell transition, although a proportion of pre-B cells survived to populate the periphery.
However, deletion of the slpA gene caused substantial alterations in cell envelope structure, and a significant defect in resistance to solvent and shear stresses compared to the wild-type.
The research group of Dr. G. D'Amico generated PTX3-deficient MSCs (PTX3−/−-MSCs) [18], which showed a significant defect in promoting tissue repair in a mice model of wound healing compared to wild-type MSCs (WT-MSCs) [18].
Darwin's twist on this response to the argument from design is to point out that it would be a particularly significant defect in the divine design of human beings if part of their very purpose in life were to suffer.
An RNAi-induced Arabidopsis mutant lacking detectable PsbP proteins exhibits a significant defect in electron transfer from QA − to QB with loss of the J to I transition of Ft, and shows seriously retarded charge recombination between QA − and OEC (Andréasson et al. 1995).
This is consistent with previous results that demonstrated a significant defect in differentiation of Wwox KO calvarial osteoblasts ex vivo compared to WT osteoblasts [16].
While most point mutations showed no significant alteration in cellular localization, one, S285I (Figure 1), showed a significant defect in nuclear localization (p<0.0001; Table 1).
ICOSL−/− mice also had a significant defect in the number of central memory phenotype CD4 T cells, but this was neither as consistent nor as dramatic as the defect observed in the EM phenotype CD4 T cell population.
