The two female backup singers were accessorized with sequined corsets and significant cleavage.
No significant cleavage of double bonds takes place.
Significant cleavage of 1 (5%) can still be observed in 0.05 M trans--cyclohexane-1,2-diamine, but not in further lower catalyst concentrations.
No significant cleavage was observed with WP1 and WP2 (data not shown).
Moreover, even at the highest levels of infection there was no significant cleavage of MTT.
Again, no significant cleavage was detected, demonstrating that the palindromic organisation of the sequence was not sufficient for cleavage.
As noted above, we observed significant cleavage of IPS1 with protein extract from patient P19 (Figure 1C).
Interestingly, a third significant cleavage site was observed for Mut1 (band c), corresponding to a cleavage downstream from a 5'-TA sequence.
Each of these catalytic nucleic acids showed significant cleavage of their 5'-end labelled substrates, although at different levels (Figure 2A C, lane 2).
However incubation of C5 with thrombin does not produce significant cleavage of C5, when analyzed on SDS-PAGE (Krarup, unpublished observation) therefore one or more intermediate proteases might be required.
