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Nine have significant alignments to other parasitic nematode ESTs, one aligns to an Onchocerca volvulus FAR protein and one aligns to a hypothetical protein from a mouse.
Sequences that produced significant alignments were extracted (using their coordinates), translated, and aligned using CLUSTALW.
Table 1 The list of fungal strains along with their respective GenBank accession number, and their identity score, that produce significant alignments with the query consensus sequence (CS) achieved from isolated Beauveria bassiana Accession no.
Like RiOG_1496, RiOG_1497 had only a few BLASTP hits with significant alignments, yet they cover a range of diverse organisms.
This protein represents the second zebrafish ortholog of Prox1, producing significant alignments (35% of identity) with all the Prox1 present in the GenBank database, including zebrafish prox1a.
BLAST analysis efficiently identifies homologous sequences through highly significant alignments.
The higher significant alignments reported were downloaded for phylogenetic analysis.
Significant alignments are then printed with their full description.
aSequences in GenBank produced significant alignments with the Yp1 sequence from skeleton 5. aSequences in our database that produced significant alignments are indicated.
Overall, 81%% of the RNA-seq contigs had significant alignments to sequences in the nt database.
A second parameter, the gap penalty, is necessary to distinguish significant alignments.
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