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Domains I and III are capable of folding into stable structural units and producing well resolved 15N/1H correlation spectra, whereas domain II forms significant aggregates at sub-millimolar concentration.
We also found that approximately 34% nascent proinsulin non-monomers appeared in 30-minute labeled human islets (Fig. 2E, F; Table S6), and that the completely folded 125I-proinsulin marker (Linco) in islet protein extracts that was subjected to the same IP and electrophoresis procedure did not form significant aggregates (Fig. S2, Table S7).
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Drying-wetting cycles cause a significant aggregate slaking, especially within the first two cycles.
Fortunately, no significant aggregate throughput drop is observed, showing that FOCA has the acceptable scalability when network becomes overloaded.
It may not seem like much, but filling these gaps can can a significant aggregate effect, given how much time we spend on our devices.
The results of multiple realizations of radionuclide transport indicate significant aggregate uncertainty in transport times through the SZ.
In general, our results suggest that the drying-wetting cycles cause a significant aggregate slaking, especially within the first two cycles.
Nevertheless, we did not detect a significant aggregate retention effect when we compared pre- and post-harvesting scenarios, although eight species decreased in abundance during the second year in control stands in both forest types.
Magnification of degraded bone area showed a significant aggregate cell marker staining with few osteoclasts (multinucleate lining cells) in the subchondral bone regions adjacent to the osteochondral interface.
However, using 100 nM fibrillar α-synucleine Bieschke et al. [ 16] found significant aggregate-bound EGCG at concentrations between 20 nM and 1 μM (apparent dissociation constant 0.1 μM).
To make an estimation of the number of cells with significant CK18 aggregates and to further confirm the relationship between CK18 aggregates and C/EBPβ 3'UTR RNA, we transfected 7721 cells with different amounts of C/EBPβ 3'UTR RNA, and then calculated [26] the percentage values of the numbers of cells with significant CK18 aggregates in the total cell populations.
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