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After the bootstrapping with 1,000 iterations, the data was found to be statistically significant (p < 0.0001) verified the significance of the clustering results.
Our group developed Monte Carlo simulation programs using a conventional personal computer with Windows XP or 2000 operating systems to examine the kinetic significance of the clustering of membrane receptors and their associate proteins and found that the pre-clustering of such proteins promotes cellular signaling [ 14].
Calculating the significance of the clustering of enriched lipids on the circular network further revealed significant clustering of nuclear-enriched lipids (p < 4.3 × 10−6), but not of mitochondrial-enriched lipids.
The OTUs that responded significantly were hierarchically clustered into groups, and the significance of the clustering verified against random clustering.
Determine significance of the clustering for at each time point.
The chi-square test was used to determine the statistical significance of the clustering.
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The level of statistical significance of the clustered risk score was statistically significant even when multiple testing (due to two outcomes) was taken into account with the conservative Bonferroni method.
For all three hormones, incorporating the covariates vIQ and age did not change the level or significance of the clusters of interest, but did attenuate the size of the significant clusters (results shown including covariates).
The resulting clusters using the k-means algorithm are compared amongst themselves as well as with the archetypical patterns of Rauber et al. For each event, statistics on wind speeds and precipitation are calculated using NCEP reanalysis data and NCEP hourly precipitation data respectively to verify the significance of the clusters.
Now that a score to evaluate the statistical significance of the clusters has been introduced, the next step is to optimize the score across the network by dividing it into proper clusters.
The main characteristic of OSLOM is that it is based on a fitness measure, the score, that is tightly related to the significance of the clusters in the configuration model.
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