Although IL-4R are expressed in a variety of tumor cells, the significance of expression of these receptors is not known.
Unlike GO miner, GSEA estimates enrichment by taking into account all of the genes with significant signals in an experiment without constrain of arbitrary cutoffs for significance of expression differences, false discovery rate and fold-change [ 13].
The significance of expression compared to WT with the P value less than 0.05 and 0.01 are marked by * and **, respectively.
The significance of expression compared to 0 h at P values of less than 0.05 and 0.01 are marked by "*" and "**", respectively.
The functional significance of expression of IL-1R1 and TNFR2 on Tregs is less clear.
The correlation significance of expression profiles between probe sets was assessed empirically by one hundred rounds of random permutations.
Student's t test was used to assess the significance of expression difference from normal and tumor tissues.
The mean and median probe signals within each annotation are calculated and the significance of expression is assessed using the binomial distribution, as in [8].
The gene expression data were normalized using MAS5 and the significance of expression of each gene in the HEK-293T cells was determined using the Affymetrix absent/present calls.
Statistical significance of expression was determined for each gene using triplicate arrays for each experimental group of BER relative to the wild type strain (ANOVA, p<0.05; Fold change ≥1.5, ≤−1.5).
To assess statistical significance of expression differences between transgenic and control cell lines, Student's T-test was carried out for control vs. transgenic groups (pair-wise, two sided, n = 6).
