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This is not a problem because neither the density-based clustering algorithm, nor the non-parametric tests of significance implemented in PROC MODECLUS have distributional assumptions.
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These are recognized through a test for statistical significance implementing the Jaccard similarity coefficient (see Section 2).
We used GO::Termfinder v0.72 [25] to identify gene ontologies that were overrepresented in the generated gene lists and considered all with an False Discovery Rate (FDR <15% significant (using simulation significances implemented in GO::Termfinder).
To identify specific differentially regulated genes between CYP2S1 depleted (759) and scrambled control (SCRAM) in human bronchial epithelial cells, we used the negative binomial test of significance as implemented in the Bioconductor package DESeq with significance defined as an adjusted p-value ≤0.05 [ 27].
When comparing the results of the two methods, statistical significance tests were performed using the suite of significance tests implemented by NIST[30].
On the other hand associations with CD for the three genes examined in this study were not noted in earlier GWA studies, at the genome-wide significance level implemented.
The Audic-Claverie significance test, implemented in the DiscoverySpace 4.0 application, was used to perform statistical analyses on the filtered tags [ 44, 46].
Significance tests implemented in GWAS software necessarily rely on assumptions, such as a lack of cryptic population structure and consistent rates of mutation across evolutionary history, that may produce higher error rates than the P-values suggest [ 41].
However, it needs to be emphasized that the number of genes predicted with multiple eQTLs could be underestimated possibly due to the small sample size and/or over-stringent significance threshold implemented in the association test.
Various statistical and multivariate analysis techniques were then used to evaluate the role and significance of implemented policies and strategies related to rabies prevention and control over this period.
Social, legal, ethical and political issues would be of equal significance and implementing global-scale projects would require a pre-existing international agreement.
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