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However, we observed a clear trend toward reduced paw swelling in mice treated with Rac1 peptide in two independent experiments, although the differences did not reach statistical significance (experiment 1: 49% reduction, P = 0.528; experiment 2: 22% reduction, P = 0.193).
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Hence, we conducted a set of statistical significance experiments to estimate the confidence of our accuracy.
In this work, different aspects of the significance of experiments related to PSE will be presented.
A Mann-Whitney test was used to determine statistical significance in experiments with two experimental groups.
Student's t-test was used to study statistical significance in experiments with only two treatments.
Statistical significance of experiments was determined using Student's t test, with a significance level of p < 0.05.
T-test was used to evaluate statistical significance of experiments used to asses KRAS mRNA and let-7 miRNA.
To determine whether R gene SNP variants are of functional significance, further experiments involving transgenic approaches, association genetic analysis or map-based cloning are required [ 72, 73].
Combined PCR data from the three biological replicates were analyzed using REST software, which normalizes results and calculates the relative fold differences in gene expression, confidence intervals and statistical significance across experiments using an integrated randomization and bootstrapping algorithm [ 30].
Despite the later significance of the experiment, cloning has never been the primary focus of the Roslin Institute.
Lawrence Norfolk and his villanelle are quite seductive and very clever, but knowingly succumbing to the lure of infidelity leads to stories that, however interesting individually (or sometimes not), by disregarding essential links to their ancestry lose significance in this experiment.
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