However, when he modified his statistical methods to take into account the fact that he was testing 24 hypotheses, not one, the boundary of significance dropped dramatically.
For example, if the removal range is further expanded to be 50 85%, the significance drops (p = 0.037).
After the top 3 (each comprising a controlling locus target gene pair, e.g., Figure 5B), significance drops off rapidly compared to background, unlike for the cis-eQTL.
Therefore, for landmarks which share the same discriminative information, we keep the landmark with the highest significance and drop others.
In the positive X and Z pools harboring mutations C170T and G221A, this is even the case with a 1/8 fraction of the data, but for the Y pools, significance levels drop to P<0.05, which is below the cut-off level P<0.01 which we considered reliable to detect true mutations.
Adjusting for STRUCTURE estimates, based on the full marker set or any of the subsets of markers ranking higher for different measures of ancestry informativeness, barely decreased the number of significant tests (Table 3), while significance levels dropped considerably (Figure S2).
The coefficient on GDP drops by almost 75% and becomes statistically indistinguishable from zero; the coefficient on schooling retains statistical significance but drops by one-half.
However, since this was a comparison with 31 statistical endpoints, the significance level dropped to p = 0.05/31 = 0.0016 by way of Bonferoni multiple end-point correction.
The significance of stress drop analysis is summarized in the next section.
Bear in mind, though, the jury is still out on the significance of that drop.
