In addition, a significance difference was obtained between free-PVX and PVX-HER (P ≤ 0.001).
Based on the mixed model, the least-square estimates of the group differences were obtained and tested for statistical significance.
Significance tests for each level of the AMOVA, the Φst values, and the pairwise differences were obtained by permuting genotypes between levels or populations.
Since Ct values reflect exponential change in the amount of mRNA, the distribution of these values around means could not be considered as normal; for these reasons no statistical significance of the differences was obtained for these values.
The significance of cancer-specific expression differences was obtained from an F-test of the variability explained by the cancer/normal variable.
No other statistically significance differences were observed.
All significance values were obtained via two-tailed tests.
Similar significance results were obtained.
The significances were obtained from the difference of the mean values between original and randomized data in terms of standard deviations of the randomized data and are also referred to as z-scores.
Each set of cross-binding assays were performed in triplicate and significance in the differences of means were obtained using a student's T-test.
