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As a result, when the PIN was divided in quintiles of significance, an analysis of the cellular component of the Gene Ontology showed a significant enrichment of nuclear proteins mostly in the 4th-5th quintiles (lowest statistical significance) (Additional file 2, Figure S3-A).
For select nutrients of potential health significance, additional sampling approaches allow for the estimation of serving-to-serving variability (e.g., highly processed foods).
The pulmonary vascular resistances tended to increase from groups 1 to 5 without reaching statistical significance (Additional file 1: Figure S11).
On the contrary, the later occurring deaths were numerically more often related to infection, renal failure, and stroke, although these differences did not reach statistical significance (Additional file 2: Table S1).
In an intention-to-treat analysis, the SF-36 data showed significant increases in social functioning (p=0.01) and in physical functioning, role limitations due to physical health, energy/fatigue, emotional well-being, pain, and general health (all p<0.03), during the diet period, although between-period differences did not reach statistical significance (Additional file 1: Table S2).
However, none of these comparisons reached regional significance (Additional file 1: Table S9).
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Please find the detailed pseudo-code for the statistical significance assessment in Additional file 8.
These results are independent of the significance threshold employed (Additional file 1).
These intervention effects were also less robust to missing data with the sensitivity analysis suggesting a trend towards non-significance (Additional file 1: Table S6b).
A congruent peak for PXO61 and PXO86 was just below the significance threshold (Additional file 3: Figure S2 and Additional file 4: Figure S3).
We recognize that, in evaluating the significance of additional steps, the § 101 patent-eligibility inquiry and, say, the § 102 novelty inquiry might sometimes overlap.
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